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A study of ligand-binding properties of Hepatocyte Nuclear Factor 4?


Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description

Hepatocyte nuclear factor 4? (HNF4?) is a liver enriched transcription factor that belongs to the nuclear receptor family, and controls the expression of genes involved in various different functions, such as nutrient transport, metabolism, growth and differentiation. Recently, fatty acids and/or fatty acyl CoAs as well as some xenobiotics (e.g. hypolipidemic drugs) have been suggested as HNF4? ligands. The aim of this study was to identify potential ligands for HNF4?, using a DAUDA fluorescence displacement assay. LFABP was expressed and purified as a positive control, and used successfully to validate the DAUDA assay. Rat HNF4?LBD was successfully expressed and purified, therefore further work for ligand identification was performed using the rat HNF4?. Suitability of the DAUDA fluorescence displacement assay for screening of ligands for rat HNF4? was confirmed by a characteristic blue shift in fluorescence emission upon protein binding, as previously reported. Ligands screened in this study included five different fatty acids, palmitoyl CoA, and hypolipidemic xenobiotics of the fibrate family (fenofibrate, bezafibrate, clofibrate), as well as pioglitazone (a thionolidinedione).

Product Specifications
SKU :COC36443
AuthorMaria Papachristodoulou,Alfred Thumser and Nick Plant
Number of Pages220
Publishing Year2/22/2012
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-31 00:00:00