Plant phenolics represent a huge number of natural compounds that are of great interest and importance nowadays due to their various pharmacological activities. The aqueous methanolic extract of Acacia Saligna was applied on Polyamide 6S and eluted with water and water/ethanol mixtures with gradual increase in concentration. The successive extraction of plant afforded the isolation of sixteen phenolic compounds, among them; Catechin, 7-O-Galloyl-Catechin, Myricetin-3-O-?-L-arabinopyranoside, Quercetin-3-O-?-D-glucopyranoside, Quercetin-3-O-?-L-arabinopyranoside, Apigenin-7-O-?-D-glucopyranoside and Luteolin-7-O-?-D-glucopyranoside which isolated for the first time from Acacia Saligna. Today, the main thrust aspect in the selection of products is safety. The best product selection must take into account non-toxic and safer starting materials as well as finished products. Whereby, the investigation showed that the dyeing and antimicrobial finishing of chrome-tanned leather with Acacia saligna leaves extract will provide an anti-candidal finishing for different leather products with good mechanical properties and fastness properties.