Aspirin (acetylsalicylic acid, ASA) has been used as one of the most famous, cheap, easily available and widely used Non Steroidal Anti Inflammatory Drug (NSAID). Aspirin is used in versatile purpose such as, anti-inflammatory (in joint diseases), anti-platelets (in cardiovascular disease), analgesic and antipyretic. The aim of present investigation is to study the effect of oral administration of drug acetylsalicylic acid (ASA, aspirin) on female albino rat, Rattus norvegicus. The female rats (n=24) were allocated into 2 groups as control (n=12) and treated (n=12). The treated rats were given oral dose of aspirin 100 mg/ kg body weight for 15 days (n=6) and 30 days (n=6). The present investigation results suggest that the high doses of ASA, induced histopathological changes in liver, kidney and reproductive organs directly or indirectly through modulation of certain biochemical and enzymological activities. These effects are dose and duration dependent.