Endau Rompin Forest reserve, Johor, Malaysia encompasses an area of about 870 km2 located at 2º 26'' 20" N, 103º 16'' 22" E. It is one of the largest national park in Peninsular Malaysia, situated at the southern tip of the peninsular. It is a nature''s heritage that housed many unique and endemic plants and animals. It also housed ancient volcanic rocks which were believed to have formed about 240 million years ago. In fact, quartz crystal ignimbrite can be clearly seen on the ground surface at the Upeh Guling water fall which is about 15 minutes walk from Kuala Merong. It is considered to be one of the last remaining lowland dipterocarp forest in Malaysia. In this manuscript some of the actinomycetes collected and identified were described. Conservation of Endau Rompin Forest Reserve in Johor, Malaysia also harbors potentially important microbes such as actinomycetes which may lead to significant discovery of secondary metabolites which may be useful in pharmaceutical, agricultural and food industries.