Category

share

AN OPTIMIZED EXPRESSION OF RECOMBINANT HEPCIDIN

AN OPTIMIZED EXPRESSION OF RECOMBINANT HEPCIDIN

 

Marketed By :  VDM Verlag Dr. Müller   Sold By :  Kamal Books International  
Delivery in :  10-12 Business Days

 
₹ 4,396

Availability: Out of stock

 

Delivery :

5% Cashback on all Orders paid using MobiKwik Wallet T&C

Free Krispy Kreme Voucher on all Orders paid using UltraCash Wallet T&C
Product Out of Stock Subscription

(Notify me when this product is back in stock)

  • Product Description
 

Expression and purification of small peptides have always been problematic due to enzymatic degradation and many other technical problems. We report cloning and expression of a low molecular weight human antimicrobial peptide ‘hepcidin'' (Hepc, 20 amino acids) in pPIC9K transformed into P. pastoris GS115. The study reveals that active hepcidin peptide can be successfully expressed in this methylotrophic yeast. The BMMY medium was found to be optimal for the hepcidin protein expression and growth of the recombinant strains. Hepcidin protein expressed in recombinant strains was about 3 mg/L. Peptide expression was verified by Western blotting and ELISA assay. Recombinant hepc 20 was purified through Reverse-Phase HPLC column and characterized by Mass Spectrometry and amino acid sequencing. It also exhibited antibacterial activity against Staphylococcus aureus and Bacillus subtilis.

Product Specifications
SKU :COC23479
Country of ManufactureIndia
Product BrandVDM Verlag Dr. Müller
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-28
0 Review(s)