Five genotypes of Brassica species were used to screen their in vitro regeneration potentiality and to establish an efficient protocol for in vitro regeneration. Different concentration and combinations of growth regulators were used in MS medium to observe the callus induction ability from anther and filament, used as explants. Among the genotypes used BARI Sarisha-7, showed the highest percentage (81.25% and 60.42%) of callus followed by Tori-7 (58.33% and 39.58%) and Agrani (41.67% and 31.25%) from filament and anther as explants. Among the treatments, highest percentage of shoot regeneration (93.75 % and 75.00%) from filament and anther were observed in MS + 4mgL-1 BAP + 1.0mgL-1 NAA (T3). BARI Sarisha-7 and Tori-7 showed better result (79.17 % & 62.50 % and 72.92% & 56.25% from filament and anther, respectively) followed by Agrani (64.58% and 47.92%) and Daulat (58.33% and 41.67%) in shoot regeneration. Highest percentage of root induction (87.50% and 75%) from filament and anther were observed in half MS + 1.0 mgL-1 IBA + 0.5mgL-1 NAA (T1). The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field.