The aim of the current study was to establish the In vitro-invivo performance after selection of vaginal modified-release dosage form. The present study concerned with the development and characterization of bioadhesive vaginal film (ZLF). Zidovudine (AZT) containing ZLF. The results demonstrate good correlations between in vitro drug release and in vivo drug absorption. Zidovudine (AZT) containing ZLF were prepared by solvent casting method using different ratios of sodium alginate, Hydroxy propyl methyl cellulose (HPMC) and Glycerol as a plasticizer. The optimized films were found to be transparent, flexible and soft and evaluated for mechanical properties by modifying the instrument, drug content, folding endurance, in-vitro and In-vivo drug release with release kinetic and % moisture content bioadhesive strength by modified pan balance method. Drug delivery through vaginal films is a promising area for continued research with the aim of achieving controlled release with enhanced bioavailability over longer periods of time. Further research, in this area will surely expected to yield a significant outcome with improved vaginal drug delivery system, for treatments of AIDS.