Plants are reservoir of effective chemotherapeutics and can provide valuable sources of natural antimicrobials. Bacteriocins are natural antimicrobial peptides with interesting potential applications in human health and exhibit a bactericidal mode of action against related as well as unrelated microorganisms. In present study Bougainvillea spectabilis and bacteriocins susceptibility against Salmonella typhi was evaluated. Bougainvillea spectabilis stem, leaves and flowers were extracted in organic solvents through soxhlet and rotary evaporator. For the bacteriocins screening, Lactococcus lactis was isolated from milk samples. Disc diffusion method was used to determine the antimicrobial activity. The results revealed that both the Bougainvillea spectabilis and bacteriocins had antimicrobial activity against S. typhi. The ethanolic and methanolic extracts of flowers and leaves of Bougainvillea spectabilis were more active against S. typhi. B. spectabilis and bacteriocins inhibition activity proved them as the alternative antimicrobial agents against human pathogens.