Chitosan(CTS) is commercially interesting polymer due to the presence of an amine group. Chitosan and its oligomers are now well known for their numerous biological properties which have led to very diverse applications. Chitosan can also be transformed into various physical forms, namely membrane, beads, fiber, solution, gel, powder and flake. Though, the processes of making chitosan films and chitosan fibers have been well studied and reported, not all of the laboratories can successfully achieve and use these techniques. On the other hands, CTS solution is easier to prepare comparing to fibers and films. It also costs less money, time, and labor needed when producing those materials of fibers, films, coated discs, etc… for the experiments. Thus, using CTS solution is a potential alternative way in cell culture, especially in researches relate to fibroblasts. Based on the matter of facts, this book focuses on the effects of CTS solution when using it in cell culture as an additive and a supplementing ingredient in culturing medium of the 3T3 fibroblast cell line and cell culture.