The experiment was conducted at Aquaculture Research and Development Centre (ARDC), Kajjansi, Wakiso district ?Uganda on the topic tolerance levels of Latesniloticus (Nile perch) on water quality parameters in glass Aquaria at Kajjansi, ARDC. The experiment was carried out for 90 days. The objectives for the research were to; determine the survival rates of Latesniloticus exposed to different levels of water quality parameters (temperature and dissolved oxygen), the lower and upper tolerance levels, the effect of DO and temperature on juveniles. Titration method was used for determining DO, PH; observation method was also used to measure temperature. Temperatures were lowered using ice and hot water of 900c was used to raise the water temperature. DO levels were altered by addition of air stones to raise DO levels and reduction of DO was done by increasing on the stocking densities. The results showed that Nile perch juveniles tolerated high temperatures up to a maximum of 36.50c and the lower temperatures were 100C. DO levels ranged between 1.9mg/l to 5.9mg/l.