In this book, the possibilities for segmentation of Helacyton gartleri cell line from their background as well as from each other in digital images from phase contrast time lapse microphotography are tested, combined and improved. Proper segmentation is one of the main issue of image evaulation and steps order differs from task to task, depending on input images. Basic terms and methods of image processing and analysis are introduced in this work. For reaching information about cell sphericity, several approaches are applied, including filtration, details emphasizing, and segmentation techniques. Sequence of selected algorithms and journey for searching them is described in details.