The research work contains standardization, Development and pharmacological evaluation of both Marketed syrup(Ayurlab's Herbal Cough Syrup) and Developed syrup. In standardisation organoleptic, physicochemical, physical and phytochemical evauation were included. Also Heavy metal and Microbial analysis and Anti oxidant activity were done. In Development water extracts of each ingredients were prepared by spray dried method(Labultima) and hot plate method. HPTLC evaluation were done by marker compounds and fingerprinting done by comparing Rf value for particular ingredient. By HPTLC evaluation it was conclude that extracts used in preparation of syrup were genuie. It contains tables that gives easy evaluation of data, figures which gives easy visulation in 254 and 366nm. It also contains 3D image and chromatogram. In pharmacological evaluation animal activity done was antitussive activity of Herbal Cough Syrup and Developed Syrup in sulphur dioxide induced cough model using mice and anti inflammatory activity of Herbal Cough Syrup and Developed Syrup in carragenan induced rat paw oedema model and statical analysis was done using Grapgpad Prism 5 software.