Soybean belongs to the family Leguminoseae and sub-family Papilionoidae and believed originated from Eastern Asia. The crop is presently grown in most of the part of world and is a primary source of vegetable oil and protein. Among the bacterial diseases bacterial blight caused by Pseudomonas syringae is causing considerable losses in soybean.The highest diseases incidence was observed in Parbhani districts and the minimum disease incidence noticed in Jalna districts. The samples collected during survey were used for isolation of pathogen. The in vitro evaluation of chemical indicated that streptocycline (100 ppm) + copper oxychlrolide (0.25%) was significant inhibited the growth of pathogen. In natural condition the streptocycline (100 ppm) + copper oxychlrolide (0.25%) was found superior over rest of treatment. In evaluation of botanicals, Neem extract significantly in habited the growth (59.26 %), the same treatment was found superior in glasshouse condition.Among the antagonist bio-agents the Trichoderma viride was significantly superior in reduction of growth of pathogen.