The somatic and genetic effects of 2.45 GHz Microwave radiation (MWR) on male and female Sprague Dawley rats were studied. One hundred and twenty rats were used for this study, grouped into control and exposed according to the parameters to study. The animals were exposed to various levels of specific absorption rate (SARs) using the microwave generator, model ER660E, Serial No MX704CCR from Toshiba UK Ltd. All animals were kept in healthy, and irradiation free environment with water and feed provided ad libitum. The study is divided into three parts; firstly, the effects of MWR on the anxiolytic and exploratory behavior of the animals. MWR produced dose-dependent significant decrease in the cumulative time spent in the open arms in the first twelve days after exposure and gradually increased towards the control value before the end of two weeks.