Tobacco (Nicotiana tabacum L.) is an important cash crop, belongs to family Solanaceae.Bidi tobacco suffers from many disease caused by a wide range of pathogens, viz., fungi, bacteria, viruses, nematodes, flowering plant parasites and phytoplasmas. Pythium spp. are essentially soil borne and possess a great problem in disease management. Damping-off disease is difficult to control by small component of action. Affected seedling collapse resulting in mass mortality of seedlings in patches. It appears at any stage after seeding in nursery. Complete failure of nursery may occur under most conducive conditions.