The exploration of biomass fuels encourages the reduction of world atmosphere pollution and global warming. In addition, the depletion of nonrenewable energy sources such as fossil fuels induces the developing technologies to harness new and renewable energy sources. Abundant of fruits waste can be reused for the bioethanol production. Hence, it can reduce pollution and waste material, thus helps in waste disposal management. In this research, nine parameters were studied that could affect the bioethanol production from mango waste. It was found that the bioethanol production was the highest at temperature of 30 0C and pH 5. Similarly, the highest ethanol concentration was obtained when 3 g/l of yeast used for fermentation. S. cerevisiae yeast was recognized as the best ethanol-producing strain. The bioethanol concentration was highest when the mango mash was hydrolyzed with pectinase enzyme, followed by hydrolysis using cellulase enzyme. It was found that the content of metal elements and viscosity in the bioethanol were under ASTM standard. Therefore, the bioethanol produced from mango waste was suitable to be used as engine fuel and environmetl pollution can be reduced.