The present study was conducted to evaluate the effect of biological control of sugarcane stem borer (Chilo infuscatellus Snellen) with an egg parasite Trichogramma chilonis in sugarcane crop. Experiment was laid out in Randomized Complete Block Design with five treatments i.e. (T1; 30,000eggs/acre, T2; 40,000eggs/acre, T3; 50,000, T4; 60,000 eggs per acre and T5; Control) having three replications each. Experiment was conducted at Chack No.109 G.B. near Jaranwala, District Faisalabad, Punjab. Data regarding mean infestation was recorded at the same time from the control plot and from the biologically treated plots at fortnightly intervals starting from July 15, 2005 to October 15, 2005. The results revealed that the infestation of sugarcane stem borer was reduced up to 84% when Trichogramma chilonis eggs were applied at the rate of 60,000 eggs per acre. So it is concluded that Trichogramma chilonis (Egg parasitized) can successfully be used for the suppression of sugarcane stem borer.