This book is an overview in biological control of Verticillium wilt which is a major disease on many crops around the world. It contains valuable information on application of a novel biofungicide (Talaromyces flavus), its different application methods and its mechanisms in controlling and combatting Verticillium dahliae and Verticillium albo-atrum, the causal agents of the disease. The crops that are coverd in this book include cotton, cucumber, potato and tomato which are very important and major crops around the world. Nowadays, production of healthy crops along with protection of the environment against hazardous contaminants are very important and critical issues in the agriculture. Biocontrol is one of the most promissing strategy which can help us to achieve the above-mentioned goals and lead us to a eco-friendly and sustainable agricultural system.