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Biosynthesis of lignans in plant species of the section Linum

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

A cDNA encoding a pinoresinol lariciresinol reductase PLR (PLR-Lp1) from a cell culture of Linum. perenne was cloned. Hairy root lines were transformed with an ihpRNAi construct to suppress PLR-Lp1 gene expression. As a second enzyme of the proposed pathway, Justicidin B 7-hydroxylase (JusB7H) was characterized from a microsomal fraction prepared from a L. perenne H suspension culture. The enzyme catalyzes the last step in the biosynthesis of Diphyllin by introducing a hydroxyl group in position 7 of Jus B. Enantiomeric purity in lignan biosynthesis is determined during the first steps, but on different levels. Seeds of Linum usitatissimum contain almost 99% (+)- and 1% (-)-Seco-diglucoside. A recombinant PLR (PLR-Lu1) from L. usitatissimum seeds converts only (-)-pinoresinol (Pino) to (+)-Seco. A second cDNA PLR-Lu2 from the leaves of L. usitatissimum was isolated. The recombinant protein converts only (+)-Pino to (-)-Seco. Therefore, the enantiomeric composition of lignans in the organs of L. usitatissimum is determined by the relative action of two PLRs with opposite enantiospecificity rather than a single enzyme with low enantiospecificity.

Product Specifications
SKU :COC36667
AuthorShiva Hemmati
LanguageEnglish
BindingPaperback
Number of Pages156
Publishing Year2012-12-10T00:00:00.000
ISBN978-3659228209
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-31 00:00:00