Catecholamine’s and Indoleamines level were studied in Striatum, hippocampus, hypothalamus, Midbrain, Pons Medulla, Cerebellum and Cerebral Cortex under alloxan-diabetic, glucose-induced-hyperglycaemic, NH4Cl or Sodium Acetoacetate-induced acidotic and insulin-treatment to all these animals, after 7, 30 and 60 days. We also studied the roles of Protein Kinases such as PKC-α, p38MAPK, CaM Kinase II and PKAII as well as S-100β/SERT on the regulation of brain biogenic amines (both In vivo and In vitro) under similar conditions. We found that Dopamine, Epinephrine, Norepinephrine, 5-HT and 5-HIAA were altered under experimental diabetes, hyperglycemic as well as acidotic conditions in some specific areas of brain which is not identical with each other suggesting that the changes observed are not generalized effect rather it is specific to the treatment regimen. The catecholamine’s and p38MAPK level were increased simultaneously while indoleamines were regulated by PKC-α, CaM Kinase II, PKAII and S100B/SERT under diabetic, hyperglycemic and acidotic conditions in some specific areas of brain. Insulin treatments reversed all these changes to normal level.