Cajanus cajan, (Pigeon pea or Red gram) finds an important place in the farming systems adopted by the small and marginal farmers in large number of developing countries. It has the potentials of multipurpose usage. It is mainly used as a human food because of its high nutritive value. It is evident that the various parts of the Cajanus cajan plant are exploited for their economic importance and it has also been to have medicinal and aromatic properties. The alternate and novel approach for the improvement of this crop is to complement the traditional breeding with tissue culture techniques to regenerate plants from single cells and organized tissues. Having known about this significance, standardization of protocols of tissue cultures, biochemical assays and molecular analysis, would serve as a prime step of the genetic engineering experiments. Therefore this text is particularly emphasized for the development of a procedure by which plants can be regenerated from single cells and organized tissues are essentially required for genetic engineering of Cajanus cajan.