The present finding deals the, isolation and identification of probiotics bacteria from the healthy shrimp to control the shrimp pathogens. The bacterial flora present in gut, gill and slime were isolated and they were tested against the shrimp pathogen. The gut of probiotics bacteria fed shrimp had minimum count of 14.89± 0.964 was observed in Pseudomonas sp. fed shrimp followed by Bacillus sp. (25.5±0.408) and a high count was observed in Aerobacterium sp. (26.16±0.849). The minimum count 20.16± 0.429 was observed in the gill of shrimp which were with in Acetobacterium followed by Bacillus sp (21.66±0.408) and a high count was observed in Pseudomonas sp (23.5±0.408). The minimum count 1.70± 0.504 was observed in the slime of shrimp which were fed with Pseudomonas followed by Acetobacterium sp. (2.466±0.410) and the maximum count was observed in Bacillus sp. (3.95± 0.732).