This thesis was submitted as fulfilment of the requirements for a Doctor of Philosophy degree. This book chronicles the trials and tribulations of a Ph.D candidate undergoing research into centromere biology. Neocentromeres are a quirk of nature and arise after structural insult to a chromosome and are thought to contribute to the evolution of species. Neocentromeres are great models for centromere biology as the underlying DNA sequence is unique, unlike typical centromeres which are full of repetitive elements. Therefore, the goal of this thesis was to characterise and describe the role of DNA methylation in centromere biology.