The ability of a cholesterol-reducing drug, simvastatin, to stimulate the formation of hydroxyapatite in an osteoblast-like cell line, was studied using Fourier transform infrared spectroscopy. Hydroxyapatite was detected using both Fourier transform infrared spectroscopy and von Kossa staining techniques a week earlier when cells were cultured in growth media supplemented with simvastatin in a tissue culture polystyrene system. Analysis of infrared spectra using area integration and curve-fitting techniques was performed to quantify the hydroxyapatite content in cells relative to the biological matrix. Infrared spectroscopy was used to quantify the mineralization process. Cells grown in a rotating bioreactor mineralized more rapidly than cells grown in tissue culture polystyrene systems.
|Number of Pages||116|
|Book Type||Chemical engineering|
|Country of Manufacture||India|
|Product Brand||VDM Verlag Dr. Müller|
|Product Packaging Info||Box|
|In The Box||1 Piece|
|Product First Available On ClickOnCare.com||2015-04-08 00:00:00|