This study was designed to compare the pharmacokinetic parameters of Levofloxacin in healthy volunteers and in human patients suffering from typhoid fever. The study was conducted in six healthy male volunteers and six male patients suffering from typhoid fever between the age of 25- 40 years. The patients were considered as group A and healthy volunteers were considered as group B. Both groups were treated with Levofloxacin 500mg tab orally. 5ml Blood samples were collected at 0, 0.25, 0.5, 1, 2, 3, 6, 12, 24, 36 & 72 hr from vein after oral administration. Plasma was separated by centrifugation at 5000 RPM and stored at -20ºC until assayed. Levofloxacin concentrations in plasma were measured by HPLC method. Calculation of all the pharmacokinetic parameters was done by software APO pharmacological analysis MW/PHARM version 3.02 by assuming bio-availability of levofloxacin after oral administration as 1. Data was analyzed by appropriate statistical methods and it was observed that there is no significant difference in pharmacokinetic parameters of Levofloxacin in healthy volunteers and in typhoid patients after oral administration.