Biofuel is an eco-friendly, alternative diesel fuel prepared from domestic renewable resources i.e. vegetable oils (edible or non-edible oil) and animal fats. India has about hundreds of species which could yield oilseeds that have enough potential for use in biodiesel production. Since India is deficient in edible oils, therefore, the non-edible oils could be the desirable source for production of bio-diesel. In such case, tree borne oilseeds (TBO’s) are the permanent importance of achieving self-sufficiency in oil seed production and as well as the expansion of green cover could only be achieved by cultivating Tree Borne Oilseeds (TBOs) in marginal lands/wasteland particularly Pongamia pinnata.