The aims of the present study were to development, validation and normalization of mathematics anxiety scales (MAS), as an instrument to measure students’ Mathematics anxiety for high schools . By using all of aspects of Mathematics anxiety a primarily questionnaire with 96 item was developed and piloted based on a sample of 2400 students. Data entry was performed using descriptive statistics, exploratory and confirmatory factor analysis and goodness of fit by SPSS and Lisrel soft wears. Analyzing the data extracted two factors for mathematics anxiety as math test anxiety and numerical anxiety. Psychometric properties of reliability and validity have shown significant results and are improved remarkably that this instrument can be considered as a suitable criterion for prospective clinical interventions. The MAS linked a communication gap between students and teachers. Through use of the scale, students, teachers and counselors are able to work together towards improving Mathematics courses and increasing Mathematical literacy.