Due to iron deficiency many complications are seen in human body.The study was conducted on 150 subjects . MCV, mean cell hemoglobin concentration Microscopic examination of Blood film .HbA1c was analysed by HPLC ( BIORAD D-10) . Serum iron & Total Binding Capacity (TIBC) Serum Ferritin it was observed that subjects those were having iron deficiency has low Hb, MCV, MCH ,Serum iron were as RDW, TIBC, UIBC and HbA1c were higher.The HbA1c is the glycation rate of RBC It has been observed that due to iron deficiency the glycation rate increases.The females are having more iron deficiency as compare to males. This is due to discrimination between the males and females and pregnancy related problems.After iron treatment significant increase was observed in the level of Hb, MCV, MCH ,Serum iron were as RDW, TIBC, UIBC and HbA1c were lower.After iron treatment level of HbA1c decreases Thus it was concluded that iron deficiency has shown marked change in the level HbA1c. The iron supplement has shown positive effect. The doctors and clinicians are advised to monitor HbA1c level when the patients are having Iron deficiency.