The present work was carried out to study the chromosomal morphology and behavior of Allium cepa L. Chromosomes. Metaphase-I of Meiosis was selected for karyotyping. It is concluded that the total length of haploid genome was 35.5 RLM.The absolute length ranges from 78-148 RLM excluding the satellite chromosome. The satellite chromosome was numbered 8, having relative length of 165 RLM and arm ratio ranging from 1.08-1.83. The arm ratio shows that the chromosomal complement consists of 4-metacentric and 3-submetacentric chromosomes.