Solving real life problems are often very time demanding due to the high sensitivity of the system such as Bio medical devices which are actually used to get data which represent the critical state of a human or animal body. There are some important indicators of human health those include ECG, SPO2, Temperature and Blood pressure. By monitoring those indicators the current state of any human health is possible to determine and hence necessary steps can be taken. This is why a Real Time monitoring of the health indicators signals has massive importance in Bio medical Engineering. In our approach we have experimentally proved and did the signal processing of important health indicator using Windows and Linux OS. For Linux we have used RT Linux which is a hard real time operating system. The data acquisition system of the signals has been done through a data acquisition card and interfacing of the system to computer has been done both in a general operating system as Windows and a Hard real time operating system RT Linux which coexists with Linux OS to make a comparison between the time dependency of signals in different operating system under multitasking environment.