This book covers general concepts of dentine bonding agents with special reference to biocompatibility. Biocompatibility of all kinds of dental materials is of paramount importance in order to prevent/limit irritation or degeneration of the surrounding tissues where it is applied. The first part of this book gives an overview of dentine bonding agents and compared the cytotoxicity of human dental pulp cell lines to a mouse 3T3 cell line to cytotoxic challenges from dentine bonding agents. The second part of the book compared the cytotoxicity of recent dentine bonding agents through artificial membranes as well as thin dentine discs (after its reaction with apatite)as well as the primer part and the bond part separately. This book covers all relevant aspects of cytotoxicity of dentine bonding agents and will be a useful guide to dentists, dental students and researchers interested in the field of biocompatibility of dental materials.