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Detection of BHV-1 infection in breeding bulls

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

Bovine herpesvirus 1 (BHV-1)is considered to be the most common viral pathogen found in bovine semen. Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine semen. A total of 89 serum samples were screened for presence of BHV-1 antibodies and 26 sera were found to be positive by M-ELISA revealing an overall seroprevalence of 29.21%. For detection viral antigen in semen, a total of 101 samples were screened by applying direct FAT of which 33 (32.67%) were found to be positive for BHV-1 antigen. The viral genome in 101 semen samples was detected by employing PCR using two sets of primers from gB and gC genes coding for viral envelope. In gB gene based PCR, 47 (46.53%) semen samples were found to be positive. While in gC gene based PCR, 43 (42.57%) were found to be positive for presence of viral genome. In sequencing of gB-gene based PCR products a consensus sequence of 459 bp was obtained. The sequences of field isolate matches completely with the sequence of BHV-1.1. Finally, the study revealed presence of BHV-1 infection in breeding bulls of Gujarat.

Product Specifications
SKU :COC89925
AuthorLata Jain and J. H. Purohit
LanguageEnglish
BindingPaperback
Number of Pages156
Publishing Year2013-03-11T00:00:00.000
ISBN9783659327612
Edition1 st
Book TypeMicrobiology (non-medical)
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-10-08 00:00:00