There is an economic importance of prawn in some developing countries. However, it has been affected by various virus and bacterial diseases in culture and hatching period respectively. Consequently, the productions of prawn have been decreasing steadily day by day. Therefore, it should be find out how production of the larvae increased in hatchery providing good health and disease free juvenile. Probiotics is one of the organic products which might increase the survival rate of larvae in hatching condition. This study shows the higher survival rate in treatment tank than in the control by using probiotics. This book, therefore, provides application of different doses of probiotics in a commercial hatchery. The observations would help to increase the survival rate providing with better water quality of culture tanks as well as raise the profitability. This book would also be useful for aquaculture consultant, potential fish farmers and entrepreneurship.