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Developing transgenic plants


Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description

Plant cell wall structural proteins (CWPs) contribute to the basal defense against pathogens. Therefore, the prospect of developing disease resistant crop cultivars through genetic manipulation of CWPs is promising. Research presented here utilized a novel CW annotated Arabidopsis gene, the PELPK1 for functional characterization by using bioinformatics and computational analyses, and by applying various reverse genetics techniques, e.g., expression, mutational, and proteomic analyses. Specific techniques used are: (i) construction of several plant transformation vectors such as PELPK1-promoter::GUS reporter fusion, PELPK1-CDS::GFP translational fusion, RNA interference of PELPK1 + PELPK2, and constitutive over-expression of PELPK1, and development of transgenic lines harbouring the above vector constructs as well as sequence-indexed T-DNA insertions in PELPK1 gene, (ii) performing of various molecular techniques such as qRT-PCR, GUS staining, confocal laser-scanning microscopy, immunolabeling, TEM, 2D gel, LCMS/MS, and microtomy, and (iii) carrying out of genotypic and phenotypic analyses of the loss of function and gain of function mutant lines under various stress conditions

Product Specifications
SKU :COC36490
AuthorAbdur Rashid
Number of Pages232
Publishing Year5/29/2012
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-31 00:00:00