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Development of Agrobacterium Mediated Transformation System for Tomato

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

This book present the work that has immense application in genetic manipulation of tomato to enhance productivity through increasing pest and disease resistance, environmental stress tolerance and to study gene function and regulation. The absence of highly efficient regeneration and transformation procedure is major obstacle in application of gene transformation technology to economically important tomato cultivars. Improved protocol for Agrobacterium mediated transformation of tomato cultivar; “Dhanshree” was developed by optimizing various parameters that affect transformation efficiency. In the present investigation Agrobacterium strain EHA 105 Harboring a pBI 121: gus gene construct was used. Cotyledons proved to be better for transformation as compared to hypocotyls and leaf explants. The best transformation efficiency was obtained with 7-14 days old explants. This transformation protocol proved to be simple, repeatable does not require any callusing phase, and may become general means of transforming tomato.

Product Specifications
SKU :COC44803
AuthorBhausaheb Pawar,Ashok Jadhav and Anil Kale
LanguageEnglish
BindingPaperback
Number of Pages84
Publishing Year2012-07-19T00:00:00.000
ISBN978-3659134715
Edition1 st
Book TypeAgriculture & farming
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-06-08 00:00:00