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Development of Molecular Markers for chromosome 5A of common wheat


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  • Product Description

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hybridization etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 5A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion lines viz; del5AL-10 and del5AL-23. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A.

Product Specifications
SKU :COC36733
AuthorAmjad Ali,Mohammad Nisar and Shah Hussain
Number of Pages56
Publishing Year2012-10-02T00:00:00.000
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-02-08 00:00:00
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