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Development of Molecular Markersfor P. Aeruginosa using RAPD

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

P. aeruginosa is considered as an opportunistic pathogen which causes infection in immunodepressed subjects. RAPD is a molecular biological technique based on PCR for revealing gene polymorphism. The product of polymorphism of RAPD is DNA fingerprint which not only shows the correlation of the development process of germline but also reflects the different distinct clones. In the study eight strains of Pseudomonas aeruginosa were characterized genotypically by RAPD analysis by comparing with the standard strain of MTCC 1749. PCR profiles revealed 6 prominent bands with molecular weights of 490bp, 520bp, 660bp, 1.3kb, 1.4kb and 1.7kb. Pair-wise co-efficient similarity based on presence and absence of bands revealed that among 8 strains, 4 strains exhibited 83% similarity, whereas, three strains showed 16.67, 50, 66.6% similarity, one strain showed 0% similarity with the standard strain. This confirms that bacteria isolated from entirely different geographical areas can also share some genetic relatedness.

Product Specifications
SKU :COC45927
AuthorAkash Srivastava and Jayant Mishra
LanguageEnglish
BindingPaperback
Number of Pages64
Publishing Year2012-06-08T00:00:00.000
ISBN978-3659131400
Edition1 st
Book TypeMicrobiology (non-medical)
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-06-08 00:00:00