Soybean rust and yellow mosaic diseases substantially reduce the yield. A Disease resistant variety developed by transfer of resistance genes in superior line is the most desirable approach. Field screening revealed significant differences among genotypes for disease reaction. Under field evaluation, none of the genotypes including rust differentials (PI 200492, PI 230970, PI 462312, ‘Ankur’, PI 459025, PI 200477, PI 230971, PI 459024, TK - 5 and Wayne) showed rust resistance, indicating evolution of new rust race(s); However, yellow mosaic (YMV) resistant varieties ‘PK 416’, ‘PK 564’, ‘PK 1024’, ‘PK 1029’, ‘SL 528’ have still maintained their resistance status. The genotypes SL 603, SL 295 remained free of YMV, while SL 328 and three derived lines from the cross PS 1024 x Ankur (F3) were highly resistant. F2 lines derived from crosses PS 1024 x Ankur, Ankur x PS 1024 and PK 416 x Samrat and genotypes Ankur, PS 1024, PS 1029, PK 416 and PS 564 were resistant. This work caters to the plant breeders and pathologist, working on the host-pathogen interactions with information on hybridization, field screening and development of disease tolerant varieties.