The primary objective of this book is to serve as a practical manual for the students of Plant Pathology. Almost all the field crops including vegetables and fruits are attacked by various categories of pathogens which have well adapted to their surroundings and occupy a wide variety of ecological niche. Hence for systematic understanding of the various aspects of the plant pathogen, the adoption of proper and relevant experimental methodology is of utmost importance. In this manual, the instructions for each practical class are presented in such a manner as to enable the students properly organize their practical exercises and acquire better understanding of the fundamentals of applied pathology. I hope this manual will be helpful for the undergraduate students and also useful to the personnel’s engaged in Plant Protection.