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Dumbelled Oligodeoxynucleotides Inhibitors of RNaseH Activity

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

Synthesis and characterization of dumbbell oligonucleotides containing loops of phosphodiester , two/ three trimethylene, C3 moieties in each loop and phosphorothioate linkages is described. Incubation of dumbbell oligonucleotides containing loops of phosphorothioate with S-1 did not result in significant cleavage. Binding of dumbbell oligonucleotides to MoMuL Virus reverse transcriptase was evaluated by PAGE band mobility shift assays. Dissociation constants evaluated using PAGE band mobility shift assays were of the order of 10-7. Investigation of inhibition of RNase H activity of reverse transcriptase showed that the dumbbell oligonucleotides containing loops of phosphorothioate are better inhibitor of the retroviral RNase H activity than the dumbbell oligonucleotides containing loops of phosphodiester , two/ three trimethylene, C3 moieties in each loop. Our studies demonstrated that the oligonucleotides containing loops of phosphorothioate displays an IC50 of 18- 25 nM in its inhibition on the reverse transcriptase RNase H activity, a magnitude lower than that of first nucleotide reverse transcriptase of HIV-1, tenofovir, introduced by Gilead Science in the market.

Product Specifications
SKU :COC36355
AuthorAjay Kumar
LanguageEnglish
BindingPaperback
Number of Pages92
Publishing Year2012-03-05T00:00:00.000
ISBN978-3659110986
Edition1 st
Book TypeBiophysics
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-31 00:00:00