This book describes, for the first time, the early and accurate pregnancy detection of water riverine buffalo using BioPRYN technology and provided the plasma levels of pregnancy-specific protein B (PSPB) using an ELISA test. The BioPRYN test differed from the other tests with earlier accuracy for detecting pregnant and non-pregnant buffaloes. Moreover, this book demonstrates the PSPB, progesterone and some biochemical parameters concentrations in amniotic fluid, allantoic fluid and fetal serum collected from slaughtered pregnant buffaloes at different months of gestation (6th, 7th and 8th). Results described the differences in PSPB and progesterone concentrations as well as blood characteristics in fetal fluids and serum during different stages of pregnancy. The placental measurements of Iraqi water buffalo during different gestation periods were also included. These findings will improve the reproductive and productive efficiency of riverine buffalo by adapting the recent management and reproductive strategies in Iraq and in the world.