The present study was elucided as a foliar spray with humic acid @ 0.05% and micronutrients like Zinc @ 0.05% and Boron @0.02% in individually and in combination on various physiological and biochemical fractions of chili (Capsicum annum Var. Longum L.). During the total experimental period two sprays were applied to plants having a gap of 15 days. The results were observed in respect to some physiological and biochemical analysis from different part and different growth phase of chili plants. Overall, it was concluded from the experiment that spraying of micronutrients such as Zinc and Boron along with humic acid were showed positive improvement in both physiological and biochemical parameters, but the most promising effect of qualitative improvement was showed in treatment with zinc only in both physiological and biochemical parameters of chili crop.