This book is primarily helpful for graduate and postgraduate students in the field of plant tissue culture, Horticulture. The objective of research was to observe the effect of different growth regulators on the regeneration behavior of Dahlia flowers. The basal Murashige and Skoog (1962) medium supplemented with various growth regulators used for shoot proliferation, callusing and further regeneration of Dahlia. Dahlia have captivating beauty is highly appreciated among the flowering bulb; a good micropropagation protocol for dahlia could be used for producing the low cost plants at higher amount. The fact of research project was initiated with an attention to optimize a protocol for micropropagation and successful regeneration of dahlia plants from both invitro and invivo sources of important indigenous and exotic cultivars of dahlia to enhance production for the local market.Mrs. Tehseen Ashraf is engaged in teaching and research in Horticulture in the University College of Agriculture, University of Sargodha for the last 4 years. Her specialization was in plant tissue culture and growth regulators and contributed 2 research papers to various National Journals.