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Effect of Induced Mutation for Varietal Improvement of Grapes

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

In vitro propagation of vitis is applied to increase plant material for cultivation. Cultures were maintained in vitro on MS medium supplemented with various growth regulators such as 2,4-D, BAP, NAA, TDZ and Kin. Comparatively all the hormones alone or in combinations were tried on grape varieties to examine their effects on initiation, as well as on multiplication. The 100% explant sterilization was achieved when free leaf explants of V1 and V2 exposed to 5% sodium hypochlorite for the duration of 5 minute. For V3 the same combination of treatment was found to be effective with 90 % survival of explants. It was observed during the present study that the nodal explants of all the three grape varieties gave 100% contamination free survival when they were treated with 5% sodium hypochlorite for 10 minutes. Best Shoot induction was observed in MS 1 medium i.e BAP 1mg/l. While maximum rooting was observed in NAA 1mg/l. The highest callus induction by leaf explants was 95% with V2 on 2.4-D 3mg/l followed by nodal segments on BA 1mg/l + NAA 0.5mg/l. Callogenesis from nodal explants regenerated in roots. By physical mutagenesis it was noted that doses up to 5 Gy helped to increase callus

Product Specifications
SKU :COC87311
AuthorIfrah Safdar,Neelma Munir and Shagufta Naz
LanguageEnglish
BindingPaperback
Number of Pages116
Publishing Year2013-08-22T00:00:00.000
ISBN9783659365997
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-10-08 00:00:00
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