Amphibians are important to the overall ecosystem balance. The large biomass of these amphibians makes them significant prey for other animals. The pesticides are widely used and have a variety of lethal and sublethal effects on non-target wildlife species. The present studies investigate the induced effects of two selected pesticide Lambda cyhalothrin (pyrethroid) and Monocrotophos (organophosphate) against frog Rana cyanophlyctis (Common Skittering Frog). After preliminary experiments of each under test compound, two different doses i.e. 0.1 and 1% were used and cholinesterase activity was noted in liver, kidney and brain of treated frogs. It was reduced upto 34.6 and 46.3% in liver, 25.08 and 57.1% in kidney and 31.64 and 50.7% in brain under the effect of lamda cyhalothrin. While under the effect of monocrotophos, cholinesterase decreased upto 37.7 and 57.7% in liver, 57.5 and 67.5% in kidney and 47.6 and 65.9% in brain, respectively. Based on present studies it is concluded that both pesticides are reduced the cholinesterase activities. The research techniques and information are helpful for graduate and research students of Environmental and Biological Sciences.