The cultivated tomato is an extremely popular and versatile vegetable crop. Bangladesh has a huge population with small land mass, which is also most vulnerable to environmental disaster due to climate change. In order to improve productivity, we need salt, flood and draught tolerant varieties, so that we can maximize our land utilization. Tomato production can be hampered by various biotic and abiotic factors,various transgenic approaches have been proved to improve tomato crop. Establishment of tissue culture protocol is a prerequisite for Agrobacterium mediated transformation experiments. This book reports a reliable and reproducible in vitro regeneration protocol avoiding complex media combination, which were developed for four local varieties. Tomato transformation efficiency is highly dependent on various transformation factors which were optimized during the present study and an efficient transformation protocol for these varieties is also reported. This book will be very helpful for the researchers interested in developing transgenic tomato for both abiotic and biotic factors or in bio-farming like production of vaccines, antibodies etc.