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Embryonic Stem Cell-like Cells in Goat (Capra hircus)

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

The objective of the present experiment was production of goat embryos either by parthenogenetic activation or by in vitro fertilization and derivation of embryonic stem (ES) cell-like cells. Firstly, the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos were investigated. Secondly, effect of cysteamine supplementation in the in vitro maturation (IVM) or culture (IVC) media, or a combination of both in terms of blastocyst production by in vitro fertilization was investigated. Finally, different methods of isolation of ES cell-like cells from in vitro produced embryos were compared and effects of Leukaemia Inhibitory Factor (LIF) and different types of feeder layers on growth of ES cell-like cells were investigated. The undifferentiated ES cell-like cells showed stem cell like morphological features, normal karyotype, expressed stem cell specific surface and intracellular markers and maintained undifferentiated state for long periods in culture. Mechanical isolation of ICMs from hatched blastocysts was found to be the best method to establish goat ES cell-like cells.

Product Specifications
SKU :COC36695
AuthorArun Kumar De and Dhruba Malakar
LanguageEnglish
BindingPaperback
Number of Pages96
Publishing Year2012-07-02T00:00:00.000
ISBN978-3846580202
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-02-08 00:00:00