The present study was carried out in 2006-7 to explore and document ethnomedicinal profile and conservation status of Plant biodiversity in "Alexander the Great Valley" Hindukash range, District Shangla, Pakistan. It is one of world''s most interesting and historical areas, not only for its natural resources and medicinal plant diversity but also for old relics or ruins of Alexander the Great that is attracting national and global interest. Study revealed that there were 32 medicinal plants belonging to 26 families. Herbaceous layer (19 species) were found to be the most used life forms followed by trees (6 species), shrubs (4 species), Climbers (2 species) and Saprophytes (1) in descending order. For knowing the ethno medicinal and socio economic profile of the people, a simple questionnaire was developed and filled through interviews. The botanical name, followed by local name, family, part used and ethnomedicinal uses in different folk was recorded. Present investigation confined to document medicinal uses of plants utilized by local peoples.