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Evaluation of antiviral and cytotoxic activity

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

The antiviral activity of plants Glyceriza glabra (roots), Phyllanthus amblicus (Fruit), Eugenia jamolana (Leaves), and Moringa oleifera (Leaves) were evaluated against Infectious bursal disease virus (IBDv) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus. Four dilutions of each extracts viz 100, 50, 25 and 12.5?g/ml were made. Vero cells were infected by identified infectious bursal disease for antiviral assay. Dilutions of these extracts were applied in triplicate manner on Vero cells (infected by IBDv), that are confluent in 96 well cell culture plates. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells by the same dilutions as used in antiviral assay and incubated the both 96 well cell culture plates for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol–2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Endpoint of these assays was measured in terms of cell survival percentage. Results were compared for qualitative variables using Chi-square technique and quantitative variables by linear regression analysis.

Product Specifications
SKU :COC95098
AuthorWaqas Ahmad,Muhammad Ashraf and Aqeel Javeed
LanguageEnglish
BindingPaperback
Number of Pages108
Publishing Year2011-10-25T00:00:00.000
ISBN978-3846521922
Edition1 st
Book TypePharmacology
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-08-14 00:00:00