This study designed to focus on the performance of File Transfer over IPv6 using Wired and Wireless technology. The methodology for this project is adopted from the spiral methodology, which is used as guidelines into creating a unique methodology that is suitable for this project. This methodology consists of five phases, which are project planning, design and development, implementation, testing and result, and the last is producing a report analysis. A Work Breakdown Structure (WBS) is created to further define the total scope of this project, with systematic approach of what needs to be done in each phase until the project is completed. We developed our own network, which is IPv6 network that consist of wired and wireless network to achieve our project goals. For monitoring the file transfer performance we using several monitoring tools such as PRTG, Colasoft Capsa and CommView. Data was collect several times to ensure their accuracy and reliability.